Part:BBa_K1021003:Design

bar (bialaphos resistance)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 545
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 54
  Illegal NgoMIV site found at 205
  Illegal NgoMIV site found at 451
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The plasmid sequence was unknown so extensive sequencing was carried out in order to extract it from its original vector, confirm BioBrick compatibility, and transfer it to pSB1C3.

Source

pBARGPE1 from the FGSC (http://www.fgsc.net/fgn/pall.html)